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ZNF202 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZNF202 Antibody (Center) AP9774C
    Western blot analysis of ZNF202 Antibody (Center) (Cat. #AP9774c) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.
  • IHC-P - ZNF202 Antibody (Center) AP9774C
    ZNF202 Antibody (Center) (Cat. #AP9774c) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF202 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ZNF202 Antibody (Center) AP9774C
    ZNF202 Antibody (Center) (Cat. #AP9774c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ZNF202 Antibody (Center) AP9774C
    Confocal immunofluorescent analysis of ZNF202 Antibody (Center) (Cat#AP9774c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • IF - ZNF202 Antibody (Center) AP9774C
    Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody (Center)(Cat#AP9774c). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession O95125
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74720 Da
Antigen Region 342-370 aa
Additional Information
Gene ID 7753
Other Names Zinc finger protein 202, Zinc finger protein with KRAB and SCAN domains 10, ZNF202, ZKSCAN10
Target/Specificity This ZNF202 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the Central region of human ZNF202.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNF202 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF202
Synonyms ZKSCAN10
Function Transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis. Also expressed in breast carcinoma cell lines
Citations (0)

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Background

ZNF202 (Zinc finger protein 202) is a transcriptional repressor of genes affecting the vascular endothelium as well as lipid metabolism and energy homeostasis. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.

References

Aberg, K., et al. Biol. Psychiatry 67(3):279-282(2010)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
Patterson, E.S., et al. Physiol. Genomics 34(3):277-284(2008)
Stene, M.C., et al. J. Am. Coll. Cardiol. 52(5):369-377(2008)
Stene, M.C., et al. J. Lipid Res. 47(5):944-952(2006)

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$ 295.00
$ 99.00
Cat# AP9774C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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