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GPR180 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GPR180 Antibody (Center) AP9787c
    GPR180 Antibody (Center) (Cat. #AP9787c) western blot analysis in WiDr,Y79,293,A2058,A375 cell line lysates (35ug/lane).This demonstrates the GPR180 antibody detected the GPR180 protein (arrow).
  • IHC-P - GPR180 Antibody (Center) AP9787c
    GPR180 Antibody (Center) (Cat. #AP9787c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPR180 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q86V85
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 119-147 aa
Additional Information
Other Names Integral membrane protein GPR180, Intimal thickness-related receptor, GPR180, ITR
Target/Specificity This GPR180 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the Central region of human GPR180.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPR180 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPR180
Synonyms ITR
Cellular Location Membrane; Multi-pass membrane protein EMBL; AK289850; BAF82539.1; -; mRNA EMBL; AL359708; CAI12412.1; -; Genomic_DNA EMBL; CH471085; EAX08944.1; -; Genomic_DNA EMBL; BC052243; AAH52243.1; -; mRNA CCDS; CCDS9472.1; - RefSeq; NP_851320.1; NM_180989.5 UniGene; Hs.439363; - ProteinModelPortal; Q86V85; - BioGrid; 127770; 4 IntAct; Q86V85; 1 STRING; 9606.ENSP00000366157; - PhosphoSitePlus; Q86V85; - BioMuta; GPR180; - DMDM; 74750449; - EPD; Q86V85; - MaxQB; Q86V85; - PaxDb; Q86V85; - PeptideAtlas; Q86V85; - PRIDE; Q86V85; - DNASU; 160897; - Ensembl; ENST00000376958; ENSP00000366157; ENSG00000152749 GeneID; 160897; - KEGG; hsa:160897; - UCSC; uc001vly.4; human CTD; 160897; - DisGeNET; 160897; - GeneCards; GPR180; - HGNC; HGNC:28899; GPR180 HPA; HPA047250; - MIM; 607787; gene neXtProt; NX_Q86V85; - OpenTargets; ENSG00000152749; - PharmGKB; PA144596429; - eggNOG; KOG4290; Eukaryota eggNOG; ENOG410XRGS; LUCA GeneTree; ENSGT00730000111205; - HOGENOM; HOG000013021; - HOVERGEN; HBG056171; - InParanoid; Q86V85; - OMA; PGLACIS; - OrthoDB; EOG091G0LC1; - PhylomeDB; Q86V85; - TreeFam; TF314975; - GenomeRNAi; 160897; - PRO; PR:Q86V85; - Proteomes; UP000005640; Chromosome 13 Bgee; ENSG00000152749; - CleanEx; HS_GPR180; - Genevisible; Q86V85; HS GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0007186; P:G-protein coupled receptor signaling pathway; IEA:InterPro GO; GO:0019236; P:response to pheromone; IEA:InterPro InterPro; IPR019336; Intimal_thickness-rel_rcpt Pfam; PF10192; GpcrRhopsn4; 1 2: Evidence at transcript level; Complete proteome; Glycoprotein; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix SIGNAL 1 22 CHAIN 23 440 Integral membrane protein GPR180 /FTId=PRO_0000239662 TRANSMEM 173 193 Helical. TRANSMEM 202 222 Helical. TRANSMEM 249 269 Helical. TRANSMEM 284 304 Helical. TRANSMEM 321 341 Helical. TRANSMEM 360 380 Helical. TRANSMEM 389 409 Helical. CARBOHYD 105 105 N-linked (GlcNAc...). CARBOHYD 110 110 N-linked (GlcNAc...). VARIANT 32 32 T -> N (in a breast cancer sample; somatic mutation) /FTId=VAR_035925 SEQUENCE 440 AA; 49395 MW; CC703269832CF8A3 CRC64; MGGLRLLAVA LTCCWWPQGS QGKTLRGSFS STAAQDAQGQ RIGHFEFHGD HALLCVRINN IAVAVGKEAK LYLFQAQEWL KLQQSSHGYS CSEKLSKAQL TMTMNQTEHN LTVSQIPSPQ TWHVFYADKY TCQDDKENSQ VEDIPFEMVL LNPDAEGNPF DHFSAGESGL HEFFFLLVLV YFVIACIYAQ SLWQAIKKGG PMHMILKVLT TALLLQAGSA LANYIHFSSY SKDGIGVPFM GSLAEFFDIA SQIQMLYLLL SLCMGWTIVR MKKSQSRPLQ WDSTPASTGI AVFIVMTQSV LLLWEQFEDI SHHSYHSHHN LAGILLIVLR ICLALSLGCG LYQIITVERS TLKREFYITF AKGCILWFLC HPVLACISVI FSDYQRDKVI TIGVILCQSV SMVILYRLFL SHSLYWEVSS LSSVTLPLTI SSGHKSRPHF
Research Areas
Citations (0)

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Background

This gene encodes a protein that is a member of the G protein-coupled receptor superfamily. This protein is produced predominantly in vascular smooth muscle cells and may play an important role in the regulation of vascular remodeling.

References

Dunham, A., et al. Nature 428(6982):522-528(2004)
Tsukada, S., et al. Circulation 107(2):313-319(2003)
Iida, A., et al. J. Hum. Genet. 48(4):170-172(2003)

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$ 295.00
$ 99.00
Cat# AP9787c
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(40 western blots)
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