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FOXG1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FOXG1 Antibody (Center) AP9793C
    All lanes : Anti-FOXG1 Antibody (Center) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 59kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IF - FOXG1 Antibody (Center) AP9793C
    Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center)(Cat#AP9793c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - FOXG1 Antibody (Center) AP9793C
    Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - FOXG1 Antibody (Center) AP9793C
    Western blot analysis of FOXG1 Antibody (Center) (Cat. #AP9793c) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.
    detail
  • FC - FOXG1 Antibody (Center) AP9793C
    FOXG1 Antibody (Center) (Cat. #AP9793c) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P55316
Other Accession P32031, Q9YHC5, Q00939, Q60987, Q90964
Reactivity Human, Mouse
Predicted Chicken, Rat, Xenopus, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52352 Da
Antigen Region 225-252 aa
Additional Information
Gene ID 2290
Other Names Forkhead box protein G1, Brain factor 1, BF-1, BF1, Brain factor 2, BF-2, BF2, hBF-2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, HFK1, Forkhead-related protein FKHL2, HFK2, Forkhead-related protein FKHL3, HFK3, FOXG1
Target/Specificity This FOXG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-252 amino acids from the Central region of human FOXG1.
Dilution WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXG1
Function Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089, ECO:0000269|PubMed:21280142}
Tissue Location Expression is restricted to the neurons of the developing telencephalon.
Research Areas
Citations ( 0 )

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of the brain and telencephalon.

References

Mencarelli, M.A., et al. J. Med. Genet. 47(1):49-53(2010)
Philippe, C., et al. J. Med. Genet. 47(1):59-65(2010)
Ariani, F., et al. Am. J. Hum. Genet. 83(1):89-93(2008)
Adesina, A.M., et al. J. Neurooncol. 85(2):111-122(2007)
Bredenkamp, N., et al. Dev. Genes Evol. 217(3):227-233(2007)

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$ 365.00
$ 140.00
Cat# AP9793C
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