CAPN3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, IHC-P, E |
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Primary Accession | P20807 |
Other Accession | Q9GLG7 |
Reactivity | Human |
Predicted | Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 94254 Da |
Antigen Region | 603-632 aa |
Gene ID | 825 |
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Other Names | Calpain-3, Calcium-activated neutral proteinase 3, CANP 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1, nCL-1, CAPN3, CANP3, CANPL3, NCL1 |
Target/Specificity | This CAPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the C-terminal region of human CAPN3. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CAPN3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CAPN3 (HGNC:1480) |
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Synonyms | CANP3, CANPL3, NCL1 |
Function | Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53 (PubMed:23357851, PubMed:27657329). |
Cellular Location | Cytoplasm. Nucleus, nucleolus |
Tissue Location | Isoform I is skeletal muscle specific. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A.
References
Charlton, R., et al. Neuromuscul. Disord. 19(7):449-457(2009)
Moretti, D., et al. Carcinogenesis 30(6):960-967(2009)
Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)
Beckmann, J.S., et al. Neuromuscul. Disord. 18(12):913-921(2008)
Shirafuji, T., et al. Rinsho Shinkeigaku 48(9):651-655(2008)
Diaz, B.G., et al. J. Biol. Chem. 279(26):27656-27666(2004)
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