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CLDN15 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CLDN15 Antibody (Center) AP9831C
    Western blot analysis of CLDN15 Antibody (Center) (Cat. #AP9831c) in K562 cell line lysates (35ug/lane). CLDN15 (arrow) was detected using the purified Pab.
  • FC - CLDN15 Antibody (Center) AP9831C
    CLDN15 Antibody (Center) (Cat. #AP9831c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56746
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24356 Da
Antigen Region 128-157 aa
Additional Information
Gene ID 24146
Other Names Claudin-15, CLDN15
Target/Specificity This CLDN15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human CLDN15.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLDN15 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLDN15
Function Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na(+), Li(+) and K(+) ions, but selects against Cl(-) ions. Plays an important role in paracellular Na(+) transport in the intestine and in Na(+) homeostasis. Required for normal Na(+)-dependent intestinal nutrient uptake.
Cellular Location Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Note=Tight junctions form continuous circumferential cell-cell contacts at the borders of apical and lateral cell membranes that seal the intercellular space and show up as strand-like structures in electron microscopy
Tissue Location Detected in colon (at protein level).
Research Areas
Citations (0)

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Plays a major role in tight junction specific obliteration of the intercellular space, through calcium independent cell adhesion activity


Van Itallie, C.M., et al. Am. J. Physiol. Renal Physiol. 285 (6), F1078-F1084 (2003)
Gonzalez-Mariscal, L., et al. Prog. Biophys. Mol. Biol. 81(1):1-44(2003)
Tsukita, S., et al. Curr. Opin. Cell Biol. 14(5):531-536(2002)
Colegio, O.R., et al. Am. J. Physiol., Cell Physiol. 283 (1), C142-C147 (2002)

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Cat# AP9831C
(40 western blots)
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