|Application ||WB, IHC-P, FC, E|
|Calculated MW||39110 Da|
|Antigen Region||1-30 aa|
|Other Names||DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40|
|Target/Specificity||This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DFFB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CAD, DFF2, DFF40|
|Function||Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.|
|Cellular Location||Cytoplasm. Nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Hanus, J., et al. Apoptosis 13(3):377-382(2008)
Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26(2008)
Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830(2007)
Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
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