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>   home   >   Products   >   Primary Antibodies   >   RT25 Antibody (C-term)   

RT25 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RT25 Antibody (C-term) AP9851b
    Western blot analysis of RT25 Antibody (C-term) (Cat. #AP9851b) in MCF-7 cell line lysates (35ug/lane). RT25 (arrow) was detected using the purified Pab.
  • IHC-P - RT25 Antibody (C-term) AP9851b
    RT25 Antibody (C-term) (Cat. #AP9851b) IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - RT25 Antibody (C-term) AP9851b
    RT25 Antibody (C-term) (Cat. #AP9851b) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P82663
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20116 Da
Antigen Region 145-173 aa
Additional Information
Gene ID 64432
Other Names 28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25
Target/Specificity This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRT25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MRPS25
Synonyms RPMS25
Cellular Location Mitochondrion.
Citations (0)

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.


Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
Hattori, A., et al. DNA Res. 7(6):357-366(2000)

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$ 295.00
$ 99.00
Cat# AP9851b
(40 western blots)
Availability: In Stock
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