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CDC45L Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | O75419 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 65569 Da |
| Antigen Region | 236-263 aa |
| Gene ID | 8318 |
|---|---|
| Other Names | Cell division control protein 45 homolog, PORC-PI-1, CDC45, CDC45L, CDC45L2 |
| Target/Specificity | This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the Central region of human CDC45L. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CDC45L Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDC45 (HGNC:1739) |
|---|---|
| Synonyms | CDC45L, CDC45L2 |
| Function | Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. |
| Cellular Location | Nucleus. Chromosome. Note=Associates with chromatin |
| Tissue Location | Widely expressed, highest levels are found in adult testis and thymus and in fetal liver |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.
References
Chowdhury, A., et al. Mol. Cell. Biol. 30(6):1495-1507(2010)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)
Ballabeni, A., et al. J. Biol. Chem. 284(5):3028-3036(2009)
Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)
Hoskins, J.M., et al. Clin. Cancer Res. 14(6):1788-1796(2008)
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