|Application ||WB, IHC-P, FC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||6109 Da|
|Antigen Region||1-30 aa|
|Other Names||Cardiac phospholamban, PLB, PLN, PLB|
|Target/Specificity||This PLB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PLB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLB-T17 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.|
|Cellular Location||Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single- pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein|
|Tissue Location||Heart muscle (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
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