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HOXA3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HOXA3 Antibody (C-term) AP9890B
    Western blot analysis of HOXA3 Antibody (C-term) (Cat. #AP9890b) in HL-60 cell line lysates (35ug/lane). HOXA3 (arrow) was detected using the purified Pab.
  • IHC-P - HOXA3 Antibody (C-term) AP9890B
    HOXA3 Antibody (C-term) (Cat. #AP9890b) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HOXA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HOXA3 Antibody (C-term) AP9890B
    HOXA3 Antibody (C-term) (Cat. #AP9890b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O43365
Other Accession O42368, P02831, O93353, Q08DG7
Reactivity Human
Predicted Bovine, Chicken, Mouse, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46369 Da
Antigen Region 413-440 aa
Additional Information
Gene ID 3200
Other Names Homeobox protein Hox-A3, Homeobox protein Hox-1E, HOXA3, HOX1E
Target/Specificity This HOXA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human HOXA3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHOXA3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HOXA3
Synonyms HOX1E
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Citations (0)

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Background

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.

References

Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Chung, N., et al. Mol. Biol. Rep. 36(2):227-235(2009)
Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006)
Manzanares, M., et al. EMBO J. 21(3):365-376(2002)

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Cat# AP9890B
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(40 western blots)
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