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Histone H3-K9 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AP9934a 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
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AP9934a-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
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Histone H3-K9 Antibody - Product Information

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC
Primary AccessionP84243
ReactivityHuman
Isotype Rabbit Ig
Calculated MW 15328 Da

Histone H3-K9 Antibody - Additional Information

Gene ID 3021
Other Names
H3F3A; H3.3A; H3F3; Histone H3.3
Target/Specificity
This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Dilution
WB~~1:1000
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
Histone H3-K9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Histone H3-K9 Antibody - Protein Information

Name H3F3A
Synonyms H3.3A, H3F3
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location
Nucleus. Chromosome.

Histone H3-K9 Antibody - Related Products

AM1151a: Phospho-Histone H3(S10) Antibody

AM1151b: Phospho-Histone H3(S10) Antibody

AM1151c: Phospho-Histone H3(S10) Antibody

AP1050a: Me2-Histone H3(K9) Antibody

AP1051a: Histone H3 Antibody(N-term)

AP1051d: Histone H3 Antibody (S10)

BP1050a: Histone H3-MeK9 Antibody Blocking Peptide

BP1051a: Histone H3 (N-term) Blocking Peptide

BP1051d: Histone H3 Antibody (S10) Blocking Peptide

BP9934a: Histone H3-K9 Antibody Blocking Peptide

AJ1354a: Histone H3.3 Antibody Phospho (pS31)

AJ1358a: Histone-H3.3 Antibody Phospho-pS28&31

Histone H3-K9 Antibody - Research Areas

Application Data

  • WB - Histone H3-K9 Antibody AP9934a

    Western blot analysis of Methyl-K-H3-K9(2Me)-4MAPS (Cat. #AP9934a) in K562 cell line lysates (35ug/lane). H3 (arrow) was detected using the purified Pab.

  • IHC - Histone H3-K9 Antibody AP9934a

    Histone H3-K9 Antibody (Cat. #AP9934a) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Histone H3-K9 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

  • FC - Histone H3-K9 Antibody AP9934a

    Histone H3-K9 Antibody (Cat. #AP9934a) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • WB - Histone H3-K9 Antibody AP9934a
  • IHC - Histone H3-K9 Antibody AP9934a
  • FC - Histone H3-K9 Antibody AP9934a

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

REFERENCES

Teng, H., et al. J. Biol. Chem. 284(39):26368-26376(2009)
Garcia, B.A., et al. J. Biol. Chem. 282(10):7641-7655(2007)
Morris, S.A., et al. J. Biol. Chem. 282(10):7632-7640(2007)
Loyola, A., et al. Mol. Cell 24(2):309-316(2006)
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)