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Histone H3-K9 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP9934a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP9934a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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Histone H3-K9 Antibody - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P84243 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15328 Da |
Histone H3-K9 Antibody - Additional info | |
| Gene ID 3021 | |
| Other Names H3F3A; H3.3A; H3F3; Histone H3.3 | |
| Target/Specificity This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Histone H3-K9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Histone H3-K9 Antibody - Protein Information | |
| Name H3F3A | |
| Synonyms H3.3A, H3F3 | |
| Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Histone H3-K9 Antibody - Related products
AM1151a: Phospho-Histone H3-S10 Antibody
AM1151b: Phospho-Histone H3-S10 Antibody
AM1151c: Phospho-Histone H3-S10 Antibody
AP1050a: Histone H3-MeK9 Antibody
AP1051d: Histone H3 Antibody (S10)
AP3667a: Phospho-H3-S10 Antibody
AP9934a: Histone H3-K9 Antibody
RI12390: H3F3B predesign siRNA
DC00525: Human H3F3B cDNA Clone
LY11083a: H3F3B Over-expression Lysate
BP1050a: Histone H3-MeK9 Antibody Blocking Peptide
BP1051a: Histone H3 (N-term) Blocking Peptide
BP1051d: Histone H3 Antibody (S10) Blocking Peptide
BP3667a: Phospho-H3-S10 Antibody Blocking Peptide
BP8890a: H3-S10 Antibody Blocking Peptide
BP9934a: Histone H3-K9 Antibody Blocking Peptide
Histone H3-K9 Antibody - Application data
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Western blot analysis of Methyl-K-H3-K9(2Me)-4MAPS (Cat. #AP9934a) in K562 cell line lysates (35ug/lane). H3 (arrow) was detected using the purified Pab.
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Histone H3-K9 Antibody (Cat. #AP9934a) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Histone H3-K9 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Histone H3-K9 Antibody (Cat. #AP9934a) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Histone H3-K9 Antibody - Research Areas
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BACKGROUND
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
REFERENCES
Teng, H., et al. J. Biol. Chem. 284(39):26368-26376(2009) Garcia, B.A., et al. J. Biol. Chem. 282(10):7641-7655(2007) Morris, S.A., et al. J. Biol. Chem. 282(10):7632-7640(2007) Loyola, A., et al. Mol. Cell 24(2):309-316(2006) Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
