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CENPM Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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|AP9955a||400 µl (40 western blots)||In Stock||$ 255.00||DISCONTINUED INQUIRE CLICK INQUIRE Add to cart|
|AP9955a-ev||80 µl (8 western blots)||In Stock||$ 95.00||DISCONTINED INQUIRE CLICK INQUIRE Add to cart|
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CENPM Antibody (C-term) - Product info
|Application||WB, IHC, FC|
|Calculated MW||19737 Da|
CENPM Antibody (C-term) - Additional info
|Gene ID 79019|
CENPM; C22orf18; ICEN39; PANE1; Centromere protein M; Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1
This CENPM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-145 amino acids from the C-terminal region of human CENPM.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
CENPM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
CENPM Antibody (C-term) - Protein Information
|Synonyms C22orf18, ICEN39, PANE1|
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres
Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Nuclear in non-confluent cells and cytoplasmic in confluent or dividing cells (By similarity). Localizes in the kinetochore domain of centromeres
Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells
CENPM Antibody (C-term) - Related products
CENPM Antibody (C-term) - Application data
Western blot analysis of CENPM Antibody (C-term) (Cat. #AP9955a) in Ramos cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.
Western blot analysis of CENPM Antibody (C-term) (Cat. #AP9955a) in mouse NIH-3T3 cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.
CENPM Antibody (C-term) (Cat. #AP9955a) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CENPM Antibody (C-term) (Cat. #AP9955a) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CENPM Antibody (C-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
CENPMe is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly.
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009) Izuta, H., et al. Genes Cells 11(6):673-684(2006) Brickner, A.G., et al. Blood 107(9):3779-3786(2006) Okada, M., et al. Nat. Cell Biol. 8(5):446-457(2006)