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TAF2 Antibody (C-Term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - TAF2 Antibody (C-Term) AP9965a
    Western blot analysis of lysates from HT-29, Jurkat, KG-1, PC-3 cell line (from left to right), using TAF2 Antibody (C-Term)(Cat. #AP9965a). AP9965a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - TAF2 Antibody (C-Term) AP9965a
    TAF2 Antibody (C-Term) (Cat. #AP9965a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF2 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - TAF2 Antibody (C-Term) AP9965a
    TAF2 Antibody (C-Term) (Cat. #AP9965a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6P1X5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 136971 Da
Antigen Region 1152-1180 aa
Additional Information
Gene ID 6873
Other Names Transcription initiation factor TFIID subunit 2, 150 kDa cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, TAF(II)150, TAFII-150, TAFII150, TAF2, CIF150, TAF2B
Target/Specificity This TAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1180 amino acids from the C-terminal region of human TAF2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF2 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF2
Synonyms CIF150, TAF2B
Function Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter.
Cellular Location Nucleus.
Tissue Location Expressed in all tissues tested.
Research Areas
Citations (0)

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Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.


Olsen, J.V., et al. Cell 127(3):635-648(2006)
Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
Guermah, M., et al. Mol. Cell 12(4):991-1001(2003)

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$ 295.00
$ 99.00
Cat# AP9965a
(40 western blots)
Availability: In Stock
Bulk Size
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