|Application ||WB, IHC-P, FC, E|
|Other Accession||Q06AV5, P41139|
|Calculated MW||16622 Da|
|Antigen Region||1-30 aa|
|Other Names||DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27, Inhibitor of DNA binding 4, Inhibitor of differentiation 4, ID4, BHLHB27|
|Target/Specificity||This ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ID4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ID4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins
Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009) Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009)
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