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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ID4 Antibody (N-term)   

ID4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ID4 Antibody (N-term) AP9977a
    Western blot analysis of ID4 Antibody (N-term) (Cat. #AP9977a) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.
  • IHC-P - ID4 Antibody (N-term) AP9977a
    ID4 Antibody (N-term) (Cat. #AP9977a) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ID4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ID4 Antibody (N-term) AP9977a
    ID4 Antibody (N-term) (Cat. #AP9977a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P47928
Other Accession Q06AV5, P41139
Reactivity Human, Mouse
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16622 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 3400
Other Names DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27, Inhibitor of DNA binding 4, Inhibitor of differentiation 4, ID4, BHLHB27
Target/Specificity This ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ID4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsID4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ID4
Synonyms BHLHB27
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins


Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009) Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009)

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Cat# AP9977a
(40 western blots)
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