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ID4 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IHC-P, FC, E |
|---|---|
| Primary Accession | P47928 |
| Other Accession | Q06AV5, P41139 |
| Reactivity | Human, Mouse |
| Predicted | Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 16622 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 3400 |
|---|---|
| Other Names | DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27, Inhibitor of DNA binding 4, Inhibitor of differentiation 4, ID4, BHLHB27 |
| Target/Specificity | This ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ID4. |
| Dilution | FC~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ID4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ID4 |
|---|---|
| Synonyms | BHLHB27 |
| Function | Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity). |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins
References
Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009) Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009)
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