|Immunogen||Human RSV Isolate, Confirmed|
|Purification||IgG fraction covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.|
|Target/Specificity||All RSV viral antigens. Reacts well with bovine isolates. Does not react with Para 1-3, Influenza A & B or Adenovirus by IFA. Negative against HEp-2 cells and WI-38 cells.|
|Storage||Short-term (up to 6 months) store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.|
|Precautions||Goat Antibody to Respiratory Syncytial Virus (RSV) Horseradish Peroxidase Conjugated is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The references listed below are for research purposes only:1. McLellan, J.S., et al., (2010), “Structure of a more antigenic site on the Respiratory Syncytial Virus fusion glycoprotein in complex with neutralizing antibody 101F, Journal of Virology, 84(23): 12236-12244.2. Bitko, V., et al., (2007), “Nonstructural Proteins of Respiratory Syncytial Virus Suppress Premature Apoptosis by an NF-kB-Dependent, Interferon-Independent Mechanism and Facilitate Virus Growth”, Journal of Virology, 81(4): 1786-1795.3. Weltzin, R., et al., (1994), “Intranasal Monoclonal Immunoglobulin A against Respiratory Syncytial Virus Protects against Upper and Lower Respiratory Tract Infections in Mice”, Antimicrobial Agents and Chemotherapy, 38(12): 2785-2791.4. Ramaswamy, M., et al., (2004), “Specific Inhibition of Type I Interferon Signal Transduction by Respiratory Syncytial Virus”, Am. J. Respir. Cell Mol. Biol., 30: 893-900.5. Gitiban, N., et al., (2005), “Chinchilla and Murine Models of Upper Respiratory Tract Infections with Respiratory Syncytial Virus”, Journal of Virology, 79(10): 6035-6042.6. Wright, P.F., et al., (2005), “Growth of Respiratory Syncytial Virus in Primary Epithelial Cells from the Human Respiratory Tract”, Journal of Virology, 79(13): 8651-8654.7. Monick, MM., et al., (2001), “Respiratory Syncytial Virus Infection Results in Activation of Multiple Protein Kinase C Isoforms Leading to Activation of Mitogen-Activated Protein Kinase”, The Journal of Immunology, 166: 2681-2687.8. Monick, MM., et al., (2005), “Activation of the Epidermal Growth Factor Receptor by Respiratory Syncytial Virus Results in Increased Inflammation and Delayed Apoptosis”, The Journal of Biological Chemistry”, 280(3): 2147-2158.
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