|Application ||WB, IP|
|Immunogen||Adeno-associated virus capsid proteins and virus particles.|
|Purification||Protein A Chromatography|
|Target/Specificity||Reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope identified a.a. 726 - a.a. 733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. AAV-2 found in human and monkey.|
|Preservative||0.09% Sodium Azide (prior to lyophilization).|
|Format||Purified, LyophilizedReconstitute with 1 mL distilled water.|
|Storage||Store lyophilized product at 2-8°C. After reconstitution, store at 2-8°C.|
|Precautions||Monoclonal Antibody to Adeno-Associated Virus (AAV), VP1, VP2 and VP3 [B1] is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The references listed below are for research purposes only:1. Wistuba, A., et al., (1995), “Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Can proteins”, J. Virol., 69, 5311-5319.2. Wistuba, A., et al., (1997), “Subcellular compartmentalization of adeno-associated virus type 2 assembly”, J. Virol., 71, 1341-1352.3. Wobus, C.A., et al., (2000), “Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection”, J. Virology, 74, 9281-9293.
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