|Application ||IP, EIA|
|Immunogen||Adeno-associated virus capsid proteins and virus particles|
|Purification||Protein A chromatography|
|Target/Specificity||Reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids that is not present in denatured capsid proteins and native but unassembled capsid proteins.|
|Preservative||0.09% Sodium Azide (prior to lyophilization)|
|Storage||Store (up to 1 year after reconstitution) at 2-8°C.|
|Precautions||Monoclonal Antibody to Adeno-associated Virus (AAV), intact particles [A20] is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The references listed below are for research purposes only.1. Wistuba, A., et al., (1995), “Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Can proteins”, J. Virol., 69. 5311-5319.2. Wistuba, A., et al., (1997), “Subcellular compartmentalization of adeno-associates virus type 2 assembly”, J. Virol., 71, 1341-1352.3. Grimm, D., et al., (1999), “Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2”, Gene Ther. 6(7), 1322-1330.4. Wobus, C.A., (2000), “Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection”, J. Virology, 74, 9281-9293.
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