|Immunogen||Purified human/rat corticotropin-releasing factor (CRF, Sigma).|
|Purification||Three sequential precipitations with 40% Ammonium Sulfate. Purified antibodies yield 1 band in native SDS-PAGE.|
|Target/Specificity||Reacts specifically with CRF. No cross-reactivity with bovine serum albumin, insulin, ovalbumin, or epidermal growth factor. The two clones (4H9 and 5D5) recognize different epitopes on CRF. Dissociation constant = 1 x 10-9M.|
|Storage||Short term (up to 14 days) store at 2-8°C. Long term, aliquot and store at -20°C to -70°C. Avoid multiple freeze/thaw cycles.|
|Precautions||Monoclonal Antibody to Corticotropin-Releasing Factor (CRF) [4H9] is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The references listed below are for research purposes only:1. Kravchenko., et al., (1994), Hybridoma 13:59-64.2. Furalev., et al., (1994), Biochem Biophys Res Com 204:823-834.
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