|Application ||WB, IF, ICC, E|
|Other Accession||AAC51127, 1848277|
|Calculated MW||62 kDa|
|Application Notes||DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 µg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||DOK1 Antibody: TP1, TLP1, p240, TROVE1, VAULT2, Docking protein 1, Downstream of tyrosine kinase 1, telomerase-associated protein 1|
|Reconstitution & Storage||DOK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||DOK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.|
|Cellular Location||Isoform 1: Cytoplasm. Nucleus.|
|Tissue Location||Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer|
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Provided below are standard protocols that you may find useful for product applications.
DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204.
Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.
Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.
Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56(dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.
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