|Application ||WB, IHC-P, E|
|Other Accession||NP_003801, 4507593|
|Calculated MW||Predicted: 32 kDa|
|Application Notes||TRAIL antibody can be used for detection of TRAIL by Western blot at 1 µg/mL dilution. Antibody can also be used for immunohistochemistry starting at 20 µg/mL.|
|Other Names||Trail Antibody: TL2, APO2L, CD253, TRAIL, Apo-2L, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, tumor necrosis factor (ligand) superfamily, member 10|
|Reconstitution & Storage||Trail antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Trail Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.|
|Cellular Location||Membrane; Single-pass type II membrane protein|
|Tissue Location||Widespread; most predominant in spleen, lung and prostate|
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Provided below are standard protocols that you may find useful for product applications.
Trail Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. TRAIL (TNF-related apoptosis-inducing ligand) is a type II membrane protein and expressed in a variety of human tissues (1,2). The death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL (3-6). Like TNF and Fas ligand, TRAIL induces apoptosis and NF-κB activation in many tissues and cells.
Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995; 3:673-82.
Pitti RM, Marsters SA, Ruppert S, et al. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996; 271:12687-90.
Pan G, O'Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL. Science; 1997; 276:111-3.
Schneider P, Thome M, Burns K, et al. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-κB. Immunity 1997; 7:831-6.
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