|Application ||WB, IF, FC, ICC, E|
|Other Accession||NP_003174, 73747889|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 93 kDa|
|Application Notes||TACE antibody can be used for detection of TACE by Western blot at 0.5 µg/mL. For immunocytochemistry use 10 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||TACE Antibody: CSVP, TACE, NISBD, ADAM18, CD156B, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, ADAM 17, ADAM metallopeptidase domain 17|
|Target/Specificity||ADAM17; 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.|
|Reconstitution & Storage||TACE antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TACE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney|
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Provided below are standard protocols that you may find useful for product applications.
TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 1997;385:729-733
Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-α. Nature 1997;385:733-736
Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-α converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74
Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4
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