|Application ||WB, IHC-P, E|
|Other Accession||P56159, 20141405|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||51 kDa|
|Application Notes||GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 1 µg/mL.|
|Other Names||GFR alpha 1 Antibody: GDNFR, RET1L, RETL1, TRNR1, GDNFRA, GFR-ALPHA-1, GDNF family receptor alpha-1, RET ligand 1, GDNF receptor alpha-1, GDNF family receptor alpha 1|
|Target/Specificity||GFRA1; GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.|
|Reconstitution & Storage||GFR alpha 1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GFR alpha 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GDNFRA, RETL1, TRNR1|
|Function||Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor|
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Provided below are standard protocols that you may find useful for product applications.
GFR alpha 1 Antibody: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Jing S, Wen D, Yu Y, et al. GDNF-induced activation of the Ret protein tyrosine kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell 1996; 85:1113-24.
Treanor JJS, Goodman L, Sauvage FD, et al. Characterization of a multicomponent receptor for GDNF. Nature 1996;82:80-83.
Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc. Natl. Acad. Sci. USA 1997; 94:6238-43.
Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4
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