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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   Caspase-9 Antibody   

Caspase-9 Antibody

     
  • IHC - Caspase-9 Antibody ASC10049
    Immunohistochemistry of TOLLIP in human brain tissue with TOLLIP antibody at 5 µg/ml.
  • WB - Caspase-9 Antibody ASC10049
    Western blot analysis of SIRT4 in (A) human and (B) mouse liver tissue lysate with SIRT4 antibody at 0.5 µg/mL
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E, IP
Primary Accession P55211
Other Accession P55211, 842
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 46 kDa

Observed: 45 kDa
Application Notes Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Additional Information
Gene ID 842
Other Names Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9, caspase 9, apoptosis-related cysteine peptidase
Target/Specificity Caspase-9 antibody was raised against a 20 amino acid peptide near the center of human Caspase-9.

The immunogen is located within amino acids 290 - 340 of Caspase-9.
Reconstitution & Storage Caspase-9 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCaspase-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP9
Synonyms MCH6
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).
Tissue Location Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Research Areas
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Background

Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

References

Duan H, Orth K, Chinnaiyan AM, et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 1996; 271:16720-4
Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, et al. The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 1996; 271:27099-106

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$ 320.00
Cat# ASC10049
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