|Application ||WB, E|
|Other Accession||AF041377, 3114593|
|Calculated MW||25 kDa|
|Application Notes||CIDE-B antibody can be used for detection of CIDE-B by Western blot 0.5 µg/mL. An approximately 25 kDa band can be detected.|
|Other Names||CIDE-B Antibody: CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B, cell death-inducing DNA fragmentation factor, alpha subunit-like effector B|
|Target/Specificity||Cideb; It has no cross activity to CIDE-A.|
|Reconstitution & Storage||CIDE-B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CIDE-B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes, and bone marrow.
Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
Inohara N, Koseki T, Chen S, Benedict MA, Nunez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4 (RD1299)
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