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CX3CL1 Antibody

     
  • WB - CX3CL1 Antibody ASC10057
    Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/mL.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IP
Primary Accession P78423
Other Accession AAB50014, 1899259
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 42203 Da
Application Notes CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 - 2 µg/mL dilution. CX3CL1 is ofen observed migrating at 80 - 100 kDa in SDS-PAGE, presumably due to post-translational modification.
Additional Information
Gene ID 6376
Other Names CX3CL1 Antibody: NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine, fractalkine, neurotactin, FKN, A-152E5.2, Fractalkine, C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1
Target/Specificity CX3CL1;
Reconstitution & Storage CX3CL1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCX3CL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CX3CL1
Synonyms FKN, NTT, SCYD1
Function Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Most abundant in the brain and heart
Research Areas
Citations (0)

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Background

CX3CL1 Antibody: Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

References

Bajetto A, Bonavia R, Barbero S, et al. Chemokines and their receptors in the central nervous system. Front. Neuroendocrinol. 2001; 22:147-84.
Umehara H, Goda S, Imai T, et al. Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol. Cell. Biol. 2001; 79:298-302.
Mizuno T, Kawanokuchi J, Numata K, et al. Production and neuroprotective functions of fractalkine in the central nervous system. Brain Res. 2003; 979:65-70.
Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. J. Biol. Chem.1998; 273:23799-804.

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$ 320.00
Cat# ASC10057
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