|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001328, 4503171|
|Calculated MW||Predicted: 39, 43 kDa |
Observed: 50 kDa
|Application Notes||CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1, chemokine (C-X3-C motif) receptor 1|
|Target/Specificity||CX3CR1; At least two isoforms of CX3CR1 are known to exist.|
|Reconstitution & Storage||CX3CR1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CX3CR1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions (PubMed:9390561, PubMed:23125415). Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro) (PubMed:9726990). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1 (PubMed:14607932).|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in lymphoid and neural tissues.|
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Provided below are standard protocols that you may find useful for product applications.
CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was initially identified as the receptor for the transmembrane molecule fractalkine, and renamed CX3CR1. CX3CR1 is known to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
Raport CJ, Schweickart VL, Eddy RL JR, et al. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995; 163:295-9
Combadiere C, Ahuja SK , and Murphy PM. Cloning, chromosomal localization, and RNA expression of a human β chemokine receptor-like gene. DNA Cell Biol. 1995;14:673-80
Combadiere C, Gao J, Tiffany HL, et al. Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem. Biophys. Res. Commun. 1998; 253:728-32
Harrison JK, Barber CM, and Lynch KR. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci. Lett. 1994; 169:85-9
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