|Application ||WB, IF, ICC, E|
|Other Accession||EAW65166, 119585570|
|Calculated MW||41482 Da|
|Application Notes||MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||MCG10 Antibody: CBP, LIP4, MCG10Alpha-CP4, poly(rC) binding protein 4|
|Reconstitution & Storage||MCG10 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||MCG10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.|
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Provided below are standard protocols that you may find useful for product applications.
MCG10 Antibody: MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.
Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA2002; 8:265-78.
Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol.2000; 20:5602-5618.
Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res.2004; 64:4171-9.
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