|Application ||WB, E|
|Other Accession||NP_060065, 19072794|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||21508 Da|
|Application Notes||KappaB ras2 antibody can be used for detection of KappaB ras1 by Western blot at 1 µg/mL.|
|Other Names||KappaB ras2 Antibody: KBRAS2, kappaB-Ras2, KBRAS2, NF-kappa-B inhibitor-interacting Ras-like protein 2, I-kappa-B-interacting Ras-like protein 2, Kappa B-Ras protein 2, NFKB inhibitor interacting Ras-like 2|
|Reconstitution & Storage||KappaB ras2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||KappaB ras2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB (By similarity).|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
KappaB ras2 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras2 and has no cross-reactivity to kappaB-ras1.
Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6.
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