|Application ||WB, IHC-P, IF, E|
|Other Accession||AF274303, 10242289|
|Calculated MW||63 kDa|
|Application Notes||CIKS antibody can be used for detection of CIKS by Western blot at 1 µg/mL. A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||CIKS Antibody: ACT1, CIKS, C6orf2, C6orf4, C6orf5, C6orf6, CANDF8, PSORS13, Adapter protein CIKS, Nuclear factor NF-kappa-B activator 1, ACT1, TRAF3 interacting protein 2|
|Reconstitution & Storage||CIKS antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CIKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C6orf2, C6orf4, C6orf5, C6orf6|
|Function||Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
CIKS Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB associates with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κB activator 1). CIKS directly interacts with IKKγ. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Iκ B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.
Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Act1, an NF-κ B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.
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