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PUMA Antibody

  • WB - PUMA Antibody ASC10175
    Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 µg/ml
  • ICC - PUMA Antibody ASC10175
    Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 µg/mL.
  • IF - PUMA Antibody ASC10175
    Immunofluorescence of PUMA in K562 cells with PUMA antibody at 2 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96PG8
Other Accession NP_055232, 15193488
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 23 kDa
Application Notes PUMA antibody can be used for detection of PUMA by Western blot at 2 µg/mL. Antibody can also be used for immunocytochemstry at 1 µg/mL. For immunofluorescence start at 2 µg/mL.
Additional Information
Gene ID 27113
Other Names PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, BCL2 binding component 3
Target/Specificity BBC3; A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
Reconstitution & Storage PUMA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsPUMA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BBC3
Synonyms PUMA
Function Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity.
Cellular Location Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. Isoform 3 is not found in mitochondria
Citations (0)

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PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.


Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94.
Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23.

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$ 310.00
Cat# ASC10175
(40 western blots)
Availability: 5-7days
Bulk Size
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