|Application ||WB, IF, ICC, E|
|Other Accession||NP_055232, 15193488|
|Calculated MW||23 kDa|
|Application Notes||PUMA antibody can be used for detection of PUMA by Western blot at 2 µg/mL. Antibody can also be used for immunocytochemstry at 1 µg/mL. For immunofluorescence start at 2 µg/mL.|
|Other Names||PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, BCL2 binding component 3|
|Target/Specificity||BBC3; A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.|
|Reconstitution & Storage||PUMA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PUMA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity.|
|Cellular Location||Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. Isoform 3 is not found in mitochondria|
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Provided below are standard protocols that you may find useful for product applications.
PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94.
Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23.
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