|Application ||WB, IHC-P, IF, E|
|Other Accession||Q8N0Z6, 67462006|
|Calculated MW||48928 Da|
|Application Notes||TTC5 antibody can be used for the detection of TTC5 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||TTC5 Antibody: Strap, Tetratricopeptide repeat protein 5, Stress-responsive activator of p300, TPR repeat protein 5, tetratricopeptide repeat domain 5|
|Reconstitution & Storage||TTC5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TTC5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein involved in p53/TP53 response that acts by regulating and mediating the assembly of multi-protein complexes. Required to facilitate the interaction between JMY and p300/EP300 and increase p53/TP53-dependent transcription and apoptosis. Prevents p53/TP53 degradation by MDM2 (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Phosphorylation at Ser-203 results in nuclear localization, while unphosphorylated protein localizes to the cytoplasm.|
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Provided below are standard protocols that you may find useful for product applications.
TTC5 Antibody: Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.
Blatch GL and Lassle M. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. BioEssays 1999; 21:932-9
Song Y and Masison DC. Independent regulation of Hsp70 and Hsp90 chaperones by Hsp70/Hsp90-organizing protein Sti1 (Hop1). J. Biol. Chem. 2005; 280:34178-85.
Yang J, Roe SM, Cliff MJ, et al. Molecular basis for TPR domain-mediated regulation of protein phosphatase 5. EMBO J. 2005; 24:1-10.
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