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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   Ipaf Antibody   

Ipaf Antibody

  • WB - Ipaf Antibody ASC10186
    Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) µg/mL, respectively.
  • ICC - Ipaf Antibody ASC10186
    Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 µg/mL.
  • IF - Ipaf Antibody ASC10186
    Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NPP4
Other Accession NP_067032, 40788015
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 110 kDa
Application Notes Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 µg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 10 µg/mL.
Additional Information
Gene ID 58484
Other Names Ipaf Antibody: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1, CLAN, UNQ6189/PRO20215, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein, NLR family, CARD domain containing 4
Target/Specificity NLRC4;
Reconstitution & Storage Ipaf antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsIpaf Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NLRC4
Synonyms CARD12, CLAN, CLAN1, IPAF
Function Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.
Cellular Location Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments
Tissue Location Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain
Research Areas
Citations (0)

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Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.


Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.
Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.

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$ 310.00
Cat# ASC10186
(40 western blots)
Availability: 5-7days
Bulk Size
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