|Application ||WB, ICC, E|
|Other Accession||NP_065129, 10017|
|Calculated MW||21973 Da|
|Application Notes||Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.|
|Other Names||Bcl-B Antibody: Boo, Diva, BCL-B, BCLB, Bcl-2-like protein 10, Anti-apoptotic protein NrH, Bcl2-L-10, BCL2-like 10 (apoptosis facilitator)|
|Target/Specificity||Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B.|
The immunogen is located within amino acids 90 - 140 of Bcl-B.
|Reconstitution & Storage||Bcl-B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Bcl-B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes cell survival. Suppresses apoptosis induced by BAX but not BAK.|
|Cellular Location||Mitochondrion. Nucleus membrane|
|Tissue Location||Widely expressed in adult tissues. Preferentially expressed in lung, liver and kidney|
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Provided below are standard protocols that you may find useful for product applications.
Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78.
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