|Application ||WB, IHC-P, IF, E|
|Other Accession||NM_030766, 13540528|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||36598 Da|
|Application Notes||Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14, BCL2-like 14 (apoptosis facilitator)|
|Target/Specificity||BCL2L14; Although antibody should react with both isoforms, only the Bcl-GS protein has been observed|
|Reconstitution & Storage||Bcl-G antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Bcl-G Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in apoptosis.|
|Cellular Location||Cytoplasm. Isoform 2: Endomembrane system. Note=Predominantly localized to cytosolic organelles|
|Tissue Location||Isoform 1 is widely expressed. Isoform 2 is testis-specific.|
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Provided below are standard protocols that you may find useful for product applications.
Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.
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