|Application ||WB, IHC-P, IF, E|
|Other Accession||CAA73532, 2094806|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 46 kDa |
Observed: 46 kDa
|Application Notes||Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||Caspase-12 (Large) Antibody: Caspase-12, CASP-12, caspase 12|
|Target/Specificity||Casp12; Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.|
|Reconstitution & Storage||Caspase-12 (Large) antibody can be stored at -20℃, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Caspase-12 Antibody (Large) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution.|
|Tissue Location||Mainly expressed in skeletal muscle and lung.|
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Provided below are standard protocols that you may find useful for product applications.
Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Nakagawa T, Zhu H, Morishima N, et al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
Van de Craen M, Vandenabeele P, Declercq W, et al. Characterization of seven murine caspase family members. FEBS Lett. 1997; 403:61-9
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