Caspase-12 Antibody (Small)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O08736 |
Other Accession | CAA73532, 2094806 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | Predicted: 46 kDa (uncleaved) Observed: 38 kDa |
Application Notes | Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. |
Gene ID | 12364 |
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Other Names | Caspase-12 Antibody (Small): Caspase-12, CASP-12, caspase 12 |
Target/Specificity | Casp12; |
Reconstitution & Storage | Caspase-12 antibody (Small) can be stored at -20℃, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | Caspase-12 Antibody (Small) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Casp12 |
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Function | Involved in the activation cascade of caspases responsible for apoptosis execution. |
Tissue Location | Mainly expressed in skeletal muscle and lung. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
References
Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.
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