ST2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | P14719 |
Other Accession | BAA02854, 17082 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 64801 Da |
Application Notes | ST2 antibody can be used for the detection of ST2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. |
Gene ID | 17082 |
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Other Names | ST2 Antibody: T1, St2, DER4, Ly84, ST2L, Fit-1, T1/ST2, St2-rs1, Ste2, Interleukin-1 receptor-like 1, Lymphocyte antigen 84, interleukin 1 receptor-like 1 |
Target/Specificity | ST2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2. This peptide is common to all three known ST2 isoforms. The immunogen is located within amino acids 40 - 90 of ST2. |
Reconstitution & Storage | ST2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | ST2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Il1rl1 |
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Synonyms | Ly84, St2, Ste2 |
Function | Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:18450470, PubMed:17675517, PubMed:29045903, PubMed:22660580). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function (By similarity). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed:34644537). |
Cellular Location | Cell membrane; Single-pass type I membrane protein |
Tissue Location | Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines Isoform A is expressed in brain astrocytes and microglia. Isoform B is expressed in brain endothelial cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ST2 Antibody: ST2 is a member of a superfamily containing the interleukin-1 receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor (TLR) 4 signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
References
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Lanahan A, Williams JB, Sanders LK, et al. Growth factor-induced delayed early response genes. Mol Cell Biol. 1992; 12:3919-29.
Sweet MJ, Leung BP, Kang D, et al. A novel pathway regulating lipopolysaccharide-induced shock by ST2/T1 via inhibition of Toll-like receptor 4 expression. J. Immunol. 2001; 166:6633-9.
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