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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ECSIT Antibody   

ECSIT Antibody

  • WB - ECSIT Antibody ASC10264
    Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
  • IHC - ECSIT Antibody ASC10264
    Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BQ95
Other Accession NP_057665, 20149633
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 49148 Da
Application Notes ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL.
Additional Information
Gene ID 51295
Other Names ECSIT Antibody: SITPEC, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC, ECSIT homolog (Drosophila)
Target/Specificity ECSIT;
Reconstitution & Storage ECSIT antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsECSIT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).
Cellular Location Cytoplasm. Nucleus. Mitochondrion
Research Areas
Citations (0)

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ECSIT Antibody: Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.


Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Janssens S and Beyaert R. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol. Cell. 2003; 11:293-302.
Sato S, Sugiyama M, Yamamoto M, et al. Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-κ B and IFN-regulatory factor-3, in the Toll-like receptor signaling. J. Immunol. 2003; 171(8):4304-10.

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$ 310.00
Cat# ASC10264
(40 western blots)
Availability: 5-7days
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