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IRS-1 Antibody

  • WB - IRS-1 Antibody ASC10324
    Western blot analysis of IRS-1 in PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 µg/mL.
  • ICC - IRS-1 Antibody ASC10324
    Immunocytochemistry of IRS-1 in P815 cells with IRS-1 antibody at 2 µg/mL.
  • IF - IRS-1 Antibody ASC10324
    Immunofluorescence of IRS-1 in P815 cells with IRS-1 antibody at 2 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35568
Other Accession P35568, 547738
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 131591 Da
Application Notes IRS-1antibody can be used for the detection of IRS-1 by Western blot at 1 - 4 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL. For immunofluorescence start at 2 µg/mL.
Additional Information
Gene ID 3667
Other Names IRS-1 Antibody: HIRS-1, Insulin receptor substrate 1, IRS-1, insulin receptor substrate 1
Target/Specificity IRS1;
Reconstitution & Storage IRS-1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsIRS-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRS1
Function May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Research Areas
Citations (0)

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IRS-1 Antibody: Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.


Giovannone B, Scaldaferri ML, Federici M, et al. Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential. Diabetes Metab. Res. Rev. 2000; 16:434-41.
Waters SB and Pessin JE. Insulin receptor substrate 1 and 2 (IRS1 and IRS2): what a tangled web we weave. Trends in Cell Biol. 1996; 6:1-4.
Ozes ON, Akca H, Mayo LD, et al. A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1. Proc. Natl. Acad. Sci. USA 2001; 98:4640-5.
Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.

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$ 310.00
Cat# ASC10324
(40 western blots)
Availability: 5-7days
Bulk Size
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