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Bit1 Antibody

  • WB - Bit1 Antibody ASC10355
    Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 µg/mL.
  • IHC - Bit1 Antibody ASC10355
    Immunohistochemistry of Bit1 in human small intestine tissue with Bit1 antibody at 10 µg/mL.
  • IF - Bit1 Antibody ASC10355
    Immunofluorescence of Bit1 in Human Small Intestine cells with Bit1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y3E5
Other Accession NP_057161, 7706351
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 19194 Da
Application Notes Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 51651
Other Names Bit1 Antibody: BIT1, PTH2, CGI-147, BIT1, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1, PTH 2, peptidyl-tRNA hydrolase 2
Target/Specificity PTRH2;
Reconstitution & Storage Bit1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsBit1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTRH2
Synonyms BIT1, PTH2
Function The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.


Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.

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$ 310.00
Cat# ASC10355
(40 western blots)
Availability: 5-7days
Bulk Size
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