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PIST Antibody

  • WB - PIST Antibody ASC10366
    Western blot analysis of PIST in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 µg/mL.
  • IHC - PIST Antibody ASC10366
    Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 µg/mL.
  • IF - PIST Antibody ASC10366
    Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HD26
Other Accession AAG00572, 9837431
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 50520 Da
Application Notes PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 µg/mL. Antibody can also be used for immunohistochemistry starting at 1 µg/mL. For immunofluorescence start at 10 µg/mL.
Additional Information
Gene ID 57120
Other Names PIST Antibody: CAL, FIG, PIST, GOPC1, dJ94G16.2, CAL, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, golgi associated PDZ and coiled-coil motif containing
Target/Specificity GOPC;
Reconstitution & Storage PIST antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsPIST Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CAL, FIG
Function Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Note=Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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PIST Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.


Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.Carcinogenesis 1993; 14:2501-5.
Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
Yu Z, Horton A, Bravin M, et al. A novel protein complex linking the δ2 glutamate receptor and autophagy: implications for neurodegeneration in Lurcher mice. Neuron 2002; 35:921-33.

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$ 310.00
Cat# ASC10366
(40 western blots)
Availability: 5-7days
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