|Application ||WB, IHC-P, IF, ICC, E|
|Other Accession||AAH66268, 51561|
|Calculated MW||21 kDa|
|Application Notes||IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Other Names||IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha, interleukin 23, alpha subunit p19|
|Target/Specificity||IL-23 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human IL-23.|
The immunogen is located within the last 50 amino acids of IL-23.
|Reconstitution & Storage||IL-23 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||IL-23 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.|
|Cellular Location||Secreted. Note=Secreted upon association with IL12B|
|Tissue Location||Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31.
Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 2000; 13:715-25.
Sheibanie AF, Tadmori I, Jing H, et al. Prostaglandin ED induces IL-23 production in bone marrow-derived dendritic cells. FASEB J. 2004; 18:1318-20.
Pirhonen J, Matikainen S, Julkunen I. Regulation of virus-induced IL-12 and IL-23 expression in human macrophages. J. Immunol. 2002; 169:5673-8.
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