|Application ||WB, E|
|Other Accession||NP_066970, 153791755|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||39 kDa|
|Application Notes||Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 µg/mL.|
|Other Names||Cyclin O Antibody: CCNU, UDG2, Cyclin-O, Cyclin O|
|Target/Specificity||CCNO; At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.|
|Reconstitution & Storage||Cyclin O antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Cyclin O Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking.|
|Cellular Location||Cytoplasm. Note=Localizes to the apical part of cytoplasm|
|Tissue Location||Present in respiratory cells (at protein level).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.
Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43.
Muller SJ and Caradonna S. Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase. Biochim. Biophys. Acta 1991; 1088:197-207.
Muller SJ and Caradonna S. Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase. J. Biol. Chem. 1993; 268:1310-9
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.