Cyclin O Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P22674 |
Other Accession | NP_066970, 153791755 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 39 kDa |
Application Notes | Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 µg/mL. |
Gene ID | 10309 |
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Other Names | Cyclin O Antibody: CCNU, UDG2, Cyclin-O, Cyclin O |
Target/Specificity | CCNO; At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms. |
Reconstitution & Storage | Cyclin O antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | Cyclin O Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CCNO (HGNC:18576) |
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Function | Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking. |
Cellular Location | Cytoplasm. Nucleus, nucleolus Note=Localizes to the apical part of cytoplasm |
Tissue Location | Present in respiratory cells (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.
References
Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43.
Muller SJ and Caradonna S. Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase. Biochim. Biophys. Acta 1991; 1088:197-207.
Muller SJ and Caradonna S. Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase. J. Biol. Chem. 1993; 268:1310-9.
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