|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003353, 6224979|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||34645 Da|
|Application Notes||UNG1 antibody can be used for the detection of UNG1 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||UNG1 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5, UNG15, DGU, Uracil-DNA glycosylase, uracil-DNA glycosylase|
|Reconstitution & Storage||UNG1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||UNG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.|
|Cellular Location||Isoform 1: Mitochondrion.|
|Tissue Location||Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells|
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Provided below are standard protocols that you may find useful for product applications.
UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.
Krokan HE, Otterlei M, Nilsen H, et al. Properties and functions of human uracil-DNA glycosylase from the UNG gene. Prog. Nucleic Acid Res. Mol. Biol. 2001; 68:365-86.
Fromm JC and Verdine GL. Base excision repair. Adv. Protein Chem. 2004; 69:1-41.
Kachhap S and Singh KK. Mitochondrial inhibition of uracil-DNA glycosylase is not mutagenic. Mol. Cancer 2004; 3:32
Imam SZ, Karahalil B, Hogue BA, et al. Mitochondrial and nuclear DNA-repair capacity of various brain regions in mouse is altered in an age-dependent manner. Neurobiol. Aging 2006; 27:1129-36.
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