|Application ||WB, IHC-P, IF, E|
|Other Accession||O60664, 68846601|
|Calculated MW||47075 Da|
|Application Notes||TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, mannose-6-phosphate receptor binding protein 1|
|Reconstitution & Storage||TIP47 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TIP47 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.|
|Cellular Location||Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.|
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Provided below are standard protocols that you may find useful for product applications.
TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
Barbero P, Bittova L, and Pfeffer SR. Visualization of Rab9-mediated vesicle transport from endosomes to the trans Golgi in living cells. J. Cell Biol. 2002; 156:511-8.
Carroll KS, Hanna J, Simon I, et al. Role of the Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 2001; 292:1373-7.
Aivazian D, Serrano RL, and Pfeffer S. TIP47 is a key effector for Rab9 localization. J. Cell Biol. 2006; 173:917-26.
Ganley IG, Carroll K, Bittova L, et al. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol. Biol. Cell 2004; 15:5420-30.
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