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Cbl Antibody

  • WB - Cbl Antibody ASC10472
    Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
  • IF - Cbl Antibody ASC10472
    Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22681
Other Accession P22681, 115855
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 99633 Da
Application Notes Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunoflourescence starting at 20 µg/mL.
Additional Information
Gene ID 867
Other Names Cbl Antibody: CBL2, NSLL, C-CBL, RNF55, FRA11B, CBL2, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Cas-Br-M (murine) ecotropic retroviral transforming sequence
Target/Specificity CBL;
Reconstitution & Storage Cbl antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCbl Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBL
Synonyms CBL2, RNF55
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Cellular Location Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane
Research Areas
Citations (0)

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Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.


Thien CBF and Langdon WY. C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses. Biochem. J. 2005; 391:153-66
Weissman AM. Themes and variations on ubiquitylation. Nat. Rev. Mol. Cell Biol. 2001; 2:169-78.
Swaminathan G and Tsygankov AY. The Cbl family of proteins: ring leaders in regulation of cell signaling. J. Cell. Physiol. 2006; 209:21-43.

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$ 310.00
Cat# ASC10472
(40 western blots)
Availability: 5-7days
Bulk Size
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