|Application ||WB, IHC-P, E|
|Other Accession||NP_065108, 9966849|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||30063 Da|
|Application Notes||TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Other Names||TIGAR Antibody: FR2BP, TIGAR, C12orf5, Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, chromosome 12 open reading frame 5|
|Reconstitution & Storage||TIGAR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TIGAR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53.|
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Provided below are standard protocols that you may find useful for product applications.
TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.
Guimaraes DP and Hainaut P. TP53: a key gene in human cancer. Biochimie2002; 84:83-93.
Corcoran CA, Huang Y, and Sheikh MS. The regulation of energy generating pathways by p53. Cancer Biol. Ther.2006; 5:1610-3.
Bensaad K, Tsuruta A, Selak MA, et al. TIGAR, a p53-inducible regulator of glycolysis and apoptosis. Cell2006; 126:107-20.
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