|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_065393, 24431935|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||129931 Da|
|Application Notes||NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 µg/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO-A, NSP-CL, Nogo-B, Nogo-C, RTN4-A, RTN4-C, RTN4-B1, RTN4-B2, NI220/250, Nbla00271, Nbla10545, KIAA0886, My043, SP1507, Reticulon-4, Foocen, Nogo protein, reticulon 4|
|Target/Specificity||RTN4; At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.|
|Reconstitution & Storage||NogoA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NogoA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. Induces the formation and stabilization of endoplasmic reticulum (ER) tubules (PubMed:25612671, PubMed:24262037). Regulates membrane morphogenesis in the ER by promoting tubular ER production. Influences NE expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins (PubMed:26906412).|
|Cellular Location||Endoplasmic reticulum membrane; Multi- pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. Co-localizes with TMEM33 at the ER sheets|
|Tissue Location||Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific|
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Provided below are standard protocols that you may find useful for product applications.
NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
Yan R, ShiQ, Hu X, et al. Reticulon proteins: emerging players in neurodegenerative diseases. Cell. Mol. Life Sci.2006; 63:877-889.
Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature2000; 403:434-9.
Schweigreiter R and Bandtlow CE. Nogo in the injured spinal cord. J. Neurotrauma2006; 3-4:384-96.
Dupuis L, Gonzalez de Aguilar JL, di Scala F, et al. Nogo provides a molecular marker for diagnosis of amyloid lateral sclerosis. Neurobiol. Dis.2002; 10:358-65.
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