ORAI3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, ICC, E |
---|---|
Primary Accession | Q9BRQ5 |
Other Accession | Q9BRQ5, 93129 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 31499 Da |
Application Notes | ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL |
Gene ID | 93129 |
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Other Names | ORAI3 Antibody: TMEM142C, TMEM142C, Protein orai-3, Transmembrane protein 142C, ORAI calcium release-activated calcium modulator 3 |
Target/Specificity | ORAI3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human ORAI3. The immunogen is located within the first 50 amino acids of ORAI3. |
Reconstitution & Storage | ORAI3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | ORAI3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ORAI3 |
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Synonyms | TMEM142C |
Function | Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1. |
Cellular Location | Cell membrane; Multi-pass membrane protein |
Tissue Location | Expressed in both naive and effector T helper cells with higher levels in effector cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.
References
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
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